The implementation of Information Technology (IT) improves patient safety and efficiency. Electronic health record is one of IT’s innovation in health care delivery. This research aimed to assess nurses’ nursing informatics skill and nurses’ acceptance toward mobile electronic nursing record application, known as PANI/the Prototype Application of Nursing Implementation. This research was conducted at a public hospital in Pekanbaru, Indonesia, with 237 participants. Data were taken by using questionnaire which was divided into three parts: general information of the participants, self-assessed nursing informatics skill and the acceptance of PANI. Prior to handing over the questionnaire, the participants were informed about PANI through leaflet and video. The study shows that the nursing informatics skill of nurses is varied, and only 11 (4.6%) participants are classified expert. The participants’ skill is related to the education level (p = 0.036) and length of work (p = 0.014). The acceptance rate of PANI is nearly 50 : 50 , the total of nurses with low acceptance toward PANI are 117 (49.4%) participants and 120 (50.6%) participants with high acceptance. Mostly, participants worry about the data safety; the data could be erased when the server is in trouble. They also thought that the application can be error that will lead to problem in documenting. However, they believed that PANI will increase the quality of health care delivery and they agreed that the electronic system should be applied sooner. In addition, 218 (92%) participants had mobile phone and agreed with using their personal gadget to do pilot project in implementing PANI. Keywords: Nursing informatics, Electronic nursing record, Health care delivery