Work fatigue is defined as the physical situation of the body, activities and weakened motivation to do a job. Age, gender, years of service, length of work are one of the factors that cause work fatigue. Based on a preliminary survey that was conducted at Kantor KUD Tani Bahagia Kulim Jaya regarding work fatigue for 10 employees, 7 people experienced work fatigue and 3 people did not experience work fatigue. This purpose of this is to determine the factors associated with work fatigue. This type of research is a quantitative research using a Cross Sectional approach. This research was conducted at Kantor KUD Tani Bahagia Kulim Jaya using a total questionnaire technique. Then analyzed univariate and bivariate using chi square test. The results showed that the factors associated with work fatigue were gender (P value = 0.024 < 0>